[Histonet] Re: mouse paws


We process paws routinely.  We haven't felt the need to deglove the skin. 
However, we decal them briefly and trim the foot pad longitudinally on 
both sides.  After trimming we complete the decaling and process them with 
a program that is slightly longer paraffin infiltration.  Trimming the 
skin off greatly expedites decaling and improves infiltration.  In case of 
highly arthritic samples it makes them small enough to fit in a cassette 
for processing.

Good luck,
Jameel Syed, BS, HT/HTL, QIHC (ASCP)
Pathology Supervisor
Abbott BioResearch Center
100 Research Drive, Worcester, MA 01605
Tel: (508)849-2862
Fax: (508)793-4895


Message: 1
Date: Tue, 05 Oct 2004 08:30:42 -0600
From: Gayle Callis 
Subject: Re: [Histonet] mouse paws
To: "joyce judge" ,
Content-Type: text/plain; charset="iso-8859-1"; format=flowed

No we leave paws intact.  However, mouse paws are extremely dense even 
though tiny,  and after decalcification, we do a longer processing 

At 05:15 AM 10/5/2004, you wrote:
>I would like too know if people that are processing mouse paws deglove or 

>process with the skin on.
>Joyce Judge
>Visen Medical
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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