[Histonet] Pizzolata method for calcium oxalate crystals

From:Gayle Callis

Are you talking about calcium oxalate crystals in tissues?  If so 
Pizzolata's method for calcium oxalate.  You can find method in Hrapchak 
and Sheehan's Theory and Practice of Histotechnology.  Our positive control 
was canine kidney from animal that had ingested antifreeze.    We also did 
a von kossa to distinguish calcium phosphate from calcium oxalate on a 
separate, adjacent section.

If you just want to see oxalate crystals in bodily fluids (urine) they have 
unique characteristics/formation under microscopic examination - 
polarized?  long time since I had to do this.  We used do this exam via 
microscope as Med techs.

  At 09:36 AM 10/13/2004, you wrote:
>Hey there,
>Does anyone know of a procedure for oxalate crystals? If so, the info
>would be greatly appreciated. Thanks, Torie
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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