[Histonet] Alternates to OCT embedding media
Caveat: Be very aware that they are NOT the same. I strongly suggest try
samples of different brands. Vendors (bless their hearts) have always
provided us with samples. Our experience (a huge eye opener) with a new
cryoembedding product compared in parallel to OCT was a huge eyeopener. We
always fill mouse intestinal lumen with cryoembedding media, also mouse
lung. The new product failed to work as villi were not supported, and
sections were compressed and torn up. When I asked vendor if their new
cryomedia had ever been tested on murine, the answer was "NO". They had
only tried it for human application. I appreciated their honesty and they
also learned something too. This is no way meant their product was
defective, it just didn't work for our research application and not
compatible for our funky mouse work.
So I strongly advise anyone to test cryoembedding media with their
applications, sectioning temperature requirements, failure to stay around
tissue during sectioning, etc, etc, to may sure you have a media optimal
for your work. We have found some seem watery, have poor sectioning or
support qualities. These products ONLY failed for us and others may not
experience the same problems. It is strictly our laboratory's choice to
stay with OCT, but we continue to try any new cryomedias available with
feedback (privately) to the vendor(s).
Have fun experimenting - it takes time but the effort pays off.
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
Bozeman MT 59717-3610
At 08:44 AM 10/12/2004, you wrote:
>I was wondering if anyone knew of alternates to OCT-brand cryo-embedding
>media. I know that there are other brands of the stuff, but it is my
>understanding that they are all basically made out of the same
>thing. Does anyone know of any other media that is used to prepare frozen
>Do you Yahoo!?
>Express yourself with Y! Messenger! Free. Download now.
>Histonet mailing list
Histonet mailing list
<< Previous Message | Next Message >>