Andrea,

The dye that you have - CI # 35780 is Sirius red F3B (CI Direct red 80),
this is the correct dye for collagen staining.
I would suggest that you use this, just because 'they' tried the stain and
it didn't work doesn't mean it doesn't!

I suspect that you will be unable to find the mythical CI 35782.

Bryan

----- Original Message -----
From: "Andrea Grantham" 
To: 
Sent: Thursday, October 30, 2003 5:08 PM
Subject: [Histonet] Sirius red


> I have a quick question about Sirius red since it is a recent topic of
> discussion on histonet.
> I was asked to do the stain and given a protocol calling for Sirius red
F3B
> (CI 35782). I have Sirius red CI 35780. They said that they have tried the
> stain using CI 35780 and it didn't work but they cannot find the other
> Sirius red with a CI# 35782 in any of the catalogs. We have tried Sigma,
> Polysciences, PolyScientific, Newcomer, and some others...
> Any suggestions where I can find it?
> Andi Grantham
> .....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
> : (office:  AHSC 4212)          P.O. Box 245044                     :
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> : (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
> :...................................................................:
>            http://www.cba.arizona.edu/histology-lab.html
>
>
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