Re: [Histonet] RE: Digital Photomicroscopy

From:"K. Bowden"

I don't have a suggestion for a camera but I have heard that for publication quality photos you need to have a 6 to 7 megapixel camera.  Then once you have solved that issue you need to have the proper distant from the camera to the scope and that is the real problem.  We are still working on that problem in our lab.

Karen Bowden
Staff Research Associate II
UCSD
La Jolla, CA

Gordon Grant wrote:
Hello histonetters,
I have an Olympus BH-2 microscope fitted with a real nice PM10AD Olympus
film camera. I have been real happy with the set up as I take a lot of
publication photos. My problem is digital is now the way to go. I
borrowed a Coolpix MDC lens adapter with a coolpix and Olympus C-5050
camera. I not very pleased with the results. Does anyone have a
recommendation for me.

Best
Gordon Grant 

-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Saturday, October 25, 2003 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet digest, Vol 1 #101 - 12 msgs

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Today's Topics:

   1. Re: Histonet digest, Vol 1 #100 - 15 msgs (amosbrooks)
   2. Re: CD 25 (Dana Settembre)
   3. Re: Moth Sperm. (Geoff McAuliffe)
   4. can someone please explain lyses (JCarpenter764@aol.com)
   5. (no subject) (Wright, Barbara (SPRI 2))
   6. CD25 (Dawson, Glen)
   7. rhodamine auto-fluor. problem (Bonnie P Whitaker)
   8. (no subject) (JCarpenter764@aol.com)
   9. RE: (no subject) (Barry R Rittman)
  10. Reality Check (Joe Nocito)
  11. Re: CD23 and BCL-6 on B5 fixed tissue (Hadi Yaziji)
  12. RE: (no subject) (Smith, Allen)

--__--__--

Message: 1
Date: Fri, 24 Oct 2003 10:46:00 -0700 (PDT)
From: amosbrooks <amosbrooks@earthlink.net>
Reply-To: amosbrooks@earthlink.net
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet digest, Vol 1 #100 - 15 msgs

Ronnie,
     We use Novocastra's CD25 (NCL CD25 305) at 1:100. We pretreat with
hot
citrate buffer (HIER) and detect with Envision.
Amos Brooks



From: "Houston, Ronnie" <Ronnie_Houston@bshsi.com>
To: "Histonet (E-mail)" <histonet@lists.utsouthwestern.edu>
Date: Thu, 23 Oct 2003 12:58:03 -0400
Subject: [Histonet] CD 25


Is anyone using CD25 on formalin-fixed, paraffin-
embedded human tissue? What clone and source are available?

Thanks
Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972
ronnie_houston@bshsi.com


--__--__--

Message: 2
Date: Fri, 24 Oct 2003 14:09:07 -0400
From: Dana Settembre <settembr@umdnj.edu>
To: Ronnie_Houston@bshsi.com, histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] CD 25

Hello Ronnie,
I use CD25 (Interleukin-2 Receptor) from Novocastra Laboratories which
is distributed by Vector Laboratories in Burlingame, CA.
I use in on FFPE human tissue.  Their clone is  4C9.  Their catalog
number is NCL-CD25-305.
Their spec sheet recommends a tonsil for a positive control.
I use it at 1:50 with Dako's Target Retrieval Solution.
Good Luck.
Dana Settembre
University Hospital-UMDNJ
Newark, NJ


  
"Houston, Ronnie" <Ronnie_Houston@bshsi.com> 10/23/2003 9:58:03 AM

        

Is anyone using CD25 on formalin-fixed, paraffin-
embedded human tissue? What clone and source are available?

Thanks
Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972
ronnie_houston@bshsi.com 

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--__--__--

Message: 3
Date: Fri, 24 Oct 2003 14:14:46 -0400
From: Geoff McAuliffe <mcauliff@umdnj.edu>
To: Bruce Abaloz <brucea@unimelb.edu.au>
Cc: HistoNet <histonet@lists.utsouthwestern.edu>,
 histonet-admin@lists.utsouthwestern.edu
Subject: Re: [Histonet] Moth Sperm.

Can you see the nucleus? If so, I don't understand the problem. Perhaps 
you are trying to stain the whole nucleated sperm one color and the 
whole anculeate sperm another? Otherwise, just stain for something found

in a nucleus and not in the cytoplasm.

Geoff

Bruce Abaloz wrote:

  
Hello Everyone,
my name is Kathryn Mcnamara & I am a PhD student at the University of
    
Melbourne, Australia. I am examining the sperm of moths. Moths have 2
types of sperm a nucleated (eupyrene) sperm and an anucleated (apyrene)
sperm. 
  
I was/AM hoping to find something that would only stain the nucleated
    
sperm so that the two can be easily differentiated with the one stain.
If you could help me it would be very much appreciated. THANKS in
advance,
  
Kathryn McNamara

Department of Zoology
University of Melbourne
Victoria, Australia 3010


 

    

  

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