Gordon,
I faced the same dilemma!
My solution was to continue using my film camera and purchase a 4000 ppi
film scanner.
This also gave me the advantage of being able to digitize existing
photomicrographs.
The results are spectacular!
Bryan
----- Original Message -----
From: "Gordon Grant"
To:
Sent: Monday, October 27, 2003 9:58 AM
Subject: [Histonet] RE: Digital Photomicroscopy
> Hello histonetters,
> I have an Olympus BH-2 microscope fitted with a real nice PM10AD Olympus
> film camera. I have been real happy with the set up as I take a lot of
> publication photos. My problem is digital is now the way to go. I
> borrowed a Coolpix MDC lens adapter with a coolpix and Olympus C-5050
> camera. I not very pleased with the results. Does anyone have a
> recommendation for me.
>
> Best
> Gordon Grant
>
> -----Original Message-----
> From: histonet-admin@lists.utsouthwestern.edu
> [mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of
> histonet-request@lists.utsouthwestern.edu
> Sent: Saturday, October 25, 2003 10:00 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: Histonet digest, Vol 1 #101 - 12 msgs
>
> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-admin@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. Re: Histonet digest, Vol 1 #100 - 15 msgs (amosbrooks)
> 2. Re: CD 25 (Dana Settembre)
> 3. Re: Moth Sperm. (Geoff McAuliffe)
> 4. can someone please explain lyses (JCarpenter764@aol.com)
> 5. (no subject) (Wright, Barbara (SPRI 2))
> 6. CD25 (Dawson, Glen)
> 7. rhodamine auto-fluor. problem (Bonnie P Whitaker)
> 8. (no subject) (JCarpenter764@aol.com)
> 9. RE: (no subject) (Barry R Rittman)
> 10. Reality Check (Joe Nocito)
> 11. Re: CD23 and BCL-6 on B5 fixed tissue (Hadi Yaziji)
> 12. RE: (no subject) (Smith, Allen)
>
> --__--__--
>
> Message: 1
> Date: Fri, 24 Oct 2003 10:46:00 -0700 (PDT)
> From: amosbrooks
> Reply-To: amosbrooks@earthlink.net
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Re: Histonet digest, Vol 1 #100 - 15 msgs
>
> Ronnie,
> We use Novocastra's CD25 (NCL CD25 305) at 1:100. We pretreat with
> hot
> citrate buffer (HIER) and detect with Envision.
> Amos Brooks
>
>
>
> From: "Houston, Ronnie"
> To: "Histonet (E-mail)"
> Date: Thu, 23 Oct 2003 12:58:03 -0400
> Subject: [Histonet] CD 25
>
>
> Is anyone using CD25 on formalin-fixed, paraffin-
> embedded human tissue? What clone and source are available?
>
> Thanks
> Ronnie Houston
> Regional Histology Operations Manager
> Bon Secours HealthPartners Laboratories
> 5801 Bremo Road
> Richmond, VA 23226
> (804) 287 7972
> ronnie_houston@bshsi.com
>
>
> --__--__--
>
> Message: 2
> Date: Fri, 24 Oct 2003 14:09:07 -0400
> From: Dana Settembre
> To: Ronnie_Houston@bshsi.com, histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] CD 25
>
> Hello Ronnie,
> I use CD25 (Interleukin-2 Receptor) from Novocastra Laboratories which
> is distributed by Vector Laboratories in Burlingame, CA.
> I use in on FFPE human tissue. Their clone is 4C9. Their catalog
> number is NCL-CD25-305.
> Their spec sheet recommends a tonsil for a positive control.
> I use it at 1:50 with Dako's Target Retrieval Solution.
> Good Luck.
> Dana Settembre
> University Hospital-UMDNJ
> Newark, NJ
>
>
> >>> "Houston, Ronnie" 10/23/2003 9:58:03 AM
> >>>
>
> Is anyone using CD25 on formalin-fixed, paraffin-
> embedded human tissue? What clone and source are available?
>
> Thanks
> Ronnie Houston
> Regional Histology Operations Manager
> Bon Secours HealthPartners Laboratories
> 5801 Bremo Road
> Richmond, VA 23226
> (804) 287 7972
> ronnie_houston@bshsi.com
>
> ________________________________________________________________________
> ________________________________________________________
> ________________________________________________________________________
> ________________________________________________________
>
> The information in this communication is intended to be confidential to
> the Individual(s) and/or Entity to whom it is addressed.
> It may contain information of a Privileged and/or Confidential nature,
> which is subject to Federal and/or State privacy regulations.
> In the event that you are not the intended recipient or the agent of
> the intended recipient, do not copy or use the information
> contained within this communication, or allow it to be read, copied or
> utilized in any manner, by any other person(s). Should
> this communication be received in error, please notify the sender
> immediately either by response e-mail or by phone at 410-442-3250,
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>
>
> --__--__--
>
> Message: 3
> Date: Fri, 24 Oct 2003 14:14:46 -0400
> From: Geoff McAuliffe
> To: Bruce Abaloz
> Cc: HistoNet ,
> histonet-admin@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Moth Sperm.
>
> Can you see the nucleus? If so, I don't understand the problem. Perhaps
> you are trying to stain the whole nucleated sperm one color and the
> whole anculeate sperm another? Otherwise, just stain for something found
>
> in a nucleus and not in the cytoplasm.
>
> Geoff
>
> Bruce Abaloz wrote:
>
> >Hello Everyone,
> >my name is Kathryn Mcnamara & I am a PhD student at the University of
> Melbourne, Australia. I am examining the sperm of moths. Moths have 2
> types of sperm a nucleated (eupyrene) sperm and an anucleated (apyrene)
> sperm.
> >I was/AM hoping to find something that would only stain the nucleated
> sperm so that the two can be easily differentiated with the one stain.
> If you could help me it would be very much appreciated. THANKS in
> advance,
> >
> >Kathryn McNamara
> >
> >Department of Zoology
> >University of Melbourne
> >Victoria, Australia 3010
> >
> >
> >
> >
>
> --
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff@umdnj.edu
> **********************************************
>
>
>
>
>
> --__--__--
>
> Message: 4
> From: JCarpenter764@aol.com
> Date: Fri, 24 Oct 2003 14:23:51 EDT
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] can someone please explain lyses
>
>
> -------------------------------1067019831
> Content-Type: text/plain; charset="US-ASCII"
> Content-Transfer-Encoding: 7bit
>
>
>
> -------------------------------1067019831
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> Content-Transfer-Encoding: quoted-printable
>
>
> charse=
> t=3Dutf-8">
>
> #fffff=
> f">
>
> -------------------------------1067019831--
>
>
> --__--__--
>
> Message: 5
> From: "Wright, Barbara (SPRI 2)"
> To: "'histonet@pathology.swmed.edu'"
> Date: Fri, 24 Oct 2003 15:20:12 -0400
> Subject: [Histonet] (no subject)
>
> This message is in MIME format. Since your mail reader does not
> understand
> this format, some or all of this message may not be legible.
>
> ------_=_NextPart_001_01C39A63.D8B02C50
> Content-Type: text/plain
>
> To all my well versed colleagues --
>
> I am in the pursuit of slide labels that are sold in 8.5X11 sheets that
> can
> be used in a laser printer. (I haven't decided to use word, excel or
> file
> maker pro formats to generate the labels). Any help would be greatly
> appreciated.
>
> Thanks,
> Barb
>
>
> Barbara Wright
> Scientist II
> Exp. Pathology & Pharmacology
> DNAX
> 901 California Avenue
> Palo Alto, CA 94304-1104
> barbara.wright2@dnax.org
>
>
>
> *********************************************************************
> This message and any attachments are solely for the intended recipient.
> If you are not the intended recipient, disclosure, copying, use or
> distribution of the information included in this message is prohibited
> -- Please immediately and permanently delete.
>
>
>
> ------_=_NextPart_001_01C39A63.D8B02C50
> Content-Type: text/html
> Content-Transfer-Encoding: quoted-printable
>
>
>
>
> charset=3Dus-ascii">
> 5.5.2653.12">
>
>
>
>
>
To all my well versed colleagues
> --
>
>
>
I am in the pursuit of slide labels
> that a=
> re sold in 8.5X11 sheets that can be used in a laser printer. (I
> have=
> n't decided to use word, excel or file maker pro formats to generate the
> la=
> bels). Any help would be greatly appreciated.
>
>
Thanks,
> Barb
>
>
>
>
Barbara
> Wright T>
> Scientist
> II >
> Exp. Pathology
> &am=
> p; Pharmacology
> DNAX
> 901 California
> Ave=
> nue
> Palo Alto, CA
> 9430=
> 4-1104
> ITC">barbara.wright2@dn=
> ax.org
>
>
>
>
> ********************************************************************* >
> This message and any attachments are solely for the intended recipient.
> If =
> you are not the intended recipient, disclosure, copying, use or
> distributio=
> n of the information included in this message is prohibited -- Please
> immed=
> iately and permanently delete.
>
>
>
> ------_=_NextPart_001_01C39A63.D8B02C50--
>
>
> --__--__--
>
> Message: 6
> From: "Dawson, Glen"
> To: histonet@lists.utsouthwestern.edu
> Date: Fri, 24 Oct 2003 15:01:36 -0500
> Subject: [Histonet] CD25
>
> Ronnie,
>
> I use CD25 (clone Tu69) from Novocastra. Cat.# NCL-CD25-305. It is for
> FFPE tissues.
>
> Good Luck,
>
> Glen Dawson
>
>
> --__--__--
>
> Message: 7
> Reply-To:
> From: "Bonnie P Whitaker"
> To: "histonet"
> Date: Fri, 24 Oct 2003 16:59:39 -0500
> Subject: [Histonet] rhodamine auto-fluor. problem
>
> This is a multi-part message in MIME format.
>
> ------=_NextPart_000_0000_01C39A50.36511310
> Content-Type: text/plain;
> charset="iso-8859-1"
> Content-Transfer-Encoding: 7bit
>
> Hi All!
>
> I trust everyone that went to Louisville had a wonderful time and
> learned a
> lot!!
>
> I have a question for you guys: A researcher here in OB/GYN is doing
> rhodamine-labeled fluorescent work (he didn't say what antibody) on
> mouse
> fallopian tube... he thought he was having a staining problem, but has
> determined that his tissue is auto-fluorescing. The tissue is frozen in
> OCT
> and fixed in acetone/methanol. What can he do to quench this?
>
> Thanks,
> Bonnie Whitaker
> University of Texas Medical School at Houston
> 6431 Fannin Street
> MSB 2.231
> Houston, Texas 77030
> Phone 713.500.6792
> Fax 713.500.0733
>
>
>
>
> ------=_NextPart_000_0000_01C39A50.36511310
> Content-Type: text/html;
> charset="iso-8859-1"
> Content-Transfer-Encoding: quoted-printable
>
>
>
> charset=3Diso-8859-1">
>
>
>
Hi All! =
>
>
size=3D2>
>
I trust everyone =
> that went to=20
> Louisville had a wonderful time and learned a lot!!
>
size=3D2>
>
I have a question =
> for you=20
> guys: A researcher here in OB/GYN is doing rhodamine-labeled =
> fluorescent=20
> work (he didn't say what antibody) on mouse fallopian tube... he thought
> =
> he was=20
> having a staining problem, but has determined that his tissue is=20
> auto-fluorescing. The tissue is frozen in OCT and fixed in=20
> acetone/methanol. What can he do to quench =
> this?
>
size=3D2>
>
size=3D2>Thanks,
>
>
Bonnie Whitaker University of Texas
> =
> Medical=20
> School at Houston 6431 Fannin Street MSB 2.231 Houston, =
> Texas =20
> 77030 Phone 713.500.6792 Fax 713.500.0733
>
>
>
> ------=_NextPart_000_0000_01C39A50.36511310--
>
>
>
> --__--__--
>
> Message: 8
> From: JCarpenter764@aol.com
> Date: Fri, 24 Oct 2003 18:29:08 EDT
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
>
> -------------------------------1067034548
> Content-Type: text/plain; charset="US-ASCII"
> Content-Transfer-Encoding: 7bit
>
> can anyone explain to me what lyses is....i have come across this term
> several times while studying for my exam.
>
> -------------------------------1067034548
> Content-Type: text/html; charset="US-ASCII"
> Content-Transfer-Encoding: quoted-printable
>
>
> charse=
> t=3Dutf-8">
>
> #fffff=
> f">can anyone explain to me what lyses is....i have come across this
> term se=
> veral times while studying for my exam.
>
> -------------------------------1067034548--
>
>
> --__--__--
>
> Message: 9
> Date: Fri, 24 Oct 2003 18:40:14 -0500
> From: "Barry R Rittman"
> To: ,
> "histonet"
> Subject: RE: [Histonet] (no subject)
>
> THlzaXMgaXMgdGhlIHByb2Nlc3Mgb2YgZGVzdHJ1Y3Rpb24gb2Ygc3Vic3RhbmNlcy4gT2Z0
> ZW4g
> dGhpcyB0ZXJtIGlzIHVzZWQgaW4gY29uanVuY3Rpb24gd2l0aCB0aGUgbWF0ZXJpYWwgYmVp
> bmcg
> bHlzZWQgZS5nLiBseXNpcyBvZiByZWQgYmxvb2QgY2VsbHMgaXMgb2Z0ZW4gZGVzY3JpYmVk
> IGFz
> IGhlbW9seXNpcy4NCkF1dG9seXNpcyBpcyB0aGUgcHJvY2VzcyB3aGVyZWJ5IGNlbGxzICJz
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> IGRlc3RydWN0Ii4gV2hlIHRpc3N1ZSBpcyByZW1vdmVkIGZyb20gaXRzIHN1cHBvcnQgaW4g
> dGhl
> IGJvZHksIHRoZSBwSCB3aXRoaW4gdGhlIGNlbGxzIGRlY3JlYXNlcyBhbmQgb25lIGVuZCBy
> ZXN1
> bHQgaXMgdGhlIHJ1cHR1cmUgb2Ygb3JnYW5lbGxlcyBrbm93biBhcyBseXNvc29tZXMuIFRo
> ZXNl
> IG9yZ2FuZWxsZXMgY29udGFpbiBhIHZhcmlldHkgb2YgZW56eW1lcyB0aGF0IHVzdWFsbHkg
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> IGluIGEgY29udHJvb2xlZCBtYW5uZXIgdG8gZGVzdHJveSBtYXRlcmlhbHMgYW5kIG9yZ2Fu
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> cyB0YWtlbiBpbnRvIHRoZSBjZWxsIGFuZCBhbHNvIG90aGVyIG9yZ2FuZWxsZXMgdGhhdCBu
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> IHRvIGJlIGJyb2tlbiBkb3duIHNvIHRoYXQgdGhlIGNvbXBvbmVudHMgY2FuIGJlIHJldXNl
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> ZiBtZWF0IG9uIGEgY291bnRlcnRvcCBmb3Igc2V2ZXJhbCBkYXlzLCBpdCBlbmRzIHVwIGJl
> aW5n
> IGxpcXVpZmllZC4NCkhvcGUgdGhpcyBoZWxwcw0KQmFycnkgUml0dG1hbg0KDQoJLS0tLS1P
> cmln
> aW5hbCBNZXNzYWdlLS0tLS0gDQoJRnJvbTogSkNhcnBlbnRlcjc2NEBhb2wuY29tIFttYWls
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> SkNhcnBlbnRlcjc2NEBhb2wuY29tXSANCglTZW50OiBGcmkgMTAvMjQvMjAwMyA1OjI5IFBN
> IA0K
> CVRvOiBoaXN0b25ldEBsaXN0cy51dHNvdXRod2VzdGVybi5lZHUgDQoJQ2M6IA0KCVN1Ympl
> Y3Q6
> IFtIaXN0b25ldF0gKG5vIHN1YmplY3QpDQoJDQoJDQoJY2FuIGFueW9uZSBleHBsYWluIHRv
> IG1l
> IHdoYXQgbHlzZXMgaXMuLi4uaSBoYXZlIGNvbWUgYWNyb3NzIHRoaXMgdGVybSBzZXZlcmFs
> IHRp
> bWVzIHdoaWxlIHN0dWR5aW5nIGZvciBteSBleGFtLg0KDQo=
>
>
> --__--__--
>
> Message: 10
> From: "Joe Nocito"
> To: "Histonet"
> Date: Fri, 24 Oct 2003 21:13:33 -0700
> Subject: [Histonet] Reality Check
>
> I want to wish everyone back from the NSH meeting. Hope you had a
> good
> time. Maybe I can attend next year.
> I have this issue that just won't go away and I would like your
> opinions.
> In the last three years, I've purchased 4 TBS waterbaths, two of
> which
> the heating element went bad. One was three years old, the other about
> two
> years. Now, I realize that TBS has a one year warranty on their
> equipment,
> but shouldn't waterbaths last longer than 3 years? I paid over $1000 per
> waterbath and they want $500 a piece to repair them. I have seen
> Boekel,
> Lab-Line and Fisher waterbaths that were older than me (we won't go
> there
> ok?) Which is to say that I'm much older than 3 years.
> Has anyone else come across this problem? I'm just waiting for the
> other 2 waterbaths to breakdown. I could see maybe one waterbath, but
> 2,
> with the same problem. I think the odds are just too high. Am I like
> in
> outer space or does this bother anyone else? Maybe it's me. I don't
> know.
> Oh, as a last comment, we do not suffer from severe power surges either,
> just in case someone would ask. Take care and thanks again for your
> input.
>
> Joe Nocito BS, HT (ASCP) QIHC
> Histology Manager
> Pathology Reference Lab
> San Antonio, Texas
>
>
>
> --__--__--
>
> Message: 11
> Date: Fri, 24 Oct 2003 21:12:16 -0700
> Cc:
> To: "Doug Geddes"
> From: Hadi Yaziji
> Subject: Re: [Histonet] CD23 and BCL-6 on B5 fixed tissue
>
> Dear Doug,
>
> B5 fixative destroys the signal of many lymphoid markers. Please advise
> your pathologists that there's no reason to use it on lymphoid cases.
>
> Best regards,
> Hadi Yaziji, M.D.
> PhenoPath Laboratories
>
> On Friday, October 24, 2003, at 08:47 AM, Doug Geddes wrote:
>
> > Looking for any information on performance of CD23 and BCL-6
> antibodies
> > on B5 fixed tissue, re - antibody suppliers, incubation times, antigen
> > retreival(?). Thanks so much in advance.
> >
> > Doug Geddes BSc, MLT
> > LHSC, London ON, Canada
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> --__--__--
>
> Message: 12
> Date: Sat, 25 Oct 2003 11:59:21 -0400
> From: "Smith, Allen"
> To:
> Cc:
> Subject: RE: [Histonet] (no subject)
>
> This is a multi-part message in MIME format.
>
> ------_=_NextPart_001_01C39B10.F4706942
> Content-Type: text/plain;
> charset="us-ascii"
> Content-Transfer-Encoding: quoted-printable
>
> Lyses is the the third person singular of the verb "to lyse". It means
> "=
> to
> cause the breakdown of." E.g., "Complement lyses bacteria," or, "Dia=
> stase
> lyses glycogen."
> Once in a blue moon, lyses is used as the plur=
> al of lysis, which is Latin
> for dissolving. In English, lysis usually m=
> eans breakdown. In chemistry,
> it means the splitting of a compound into=
> two parts. In biology, it means
> the death of a cell and the disappeara=
> nce of its parts.
>
> Allen A. Smith, Ph.D.
> Professor of Anatomy
> School=
> of Graduate Medical Sciences
> Barry University
> Miami Shores, FL
>
> --=
> ---Original Message-----
> From: JCarpenter764@aol.com [mailto:JCarpenter=
> 764@aol.com]
> Sent: Friday, October 24, 2003 6:29 PM
> To: histonet@lis=
> ts.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
>
> can anyo=
> ne explain to me what lyses is....i have come across this
> term several t=
> imes while studying for my exam.
>
>
> =
>
>
> The information transmitted is intended only for the =
> person or entity to which it is addressed and may contain confidential,
> a=
> nd/or privileged material. No confidentiality or privilege is waived or
> l=
> ost by any errant transmission. If you receive this message in error,
> ple=
> ase immediately delete it and all copies of it from your system and
> notif=
> y the sender. E-mail transmission cannot be guaranteed to be secure or
> e=
> rror-free as information could be intercepted, corrupted, lost,
> destroyed=
> , arrive late or incomplete, or contain viruses.
> Barry University - Miam=
> i Shores, FL (http://www.barry.edu)
>
> ------_=_NextPart_001_01C39B10.F4706942
> Content-Type: text/html;
> charset="us-ascii"
> Content-Transfer-Encoding: quoted-printable
>
>
> AD>Message
> t/html; charset=3Dus-ascii">
> e=3DGENERATOR>
> ; BACKGROUND-COLOR: #ffffff">
>
T color=3D#0000ff size=3D4>Lyses is the the
> third person singular of th=
> e verb "to lyse". It means "to cause the
> breakdown of." E.g=
> ., "Complement lyses bacteria," or, "Diastase lyses
> glycogen." FONT>
>
=3D421064715-25102003>Once in a
> blue moon, l 715-25102003>yses is used as the
> plural of lysis, which is Latin =
> for dissolving. In English, lysis usually
> means breakdown. =
> In chemistry, it means the splitting of a compound into
> two parts. =
> ; In biology, it means the death of a cell and the disappearance
> of its=
> parts.
>
r=3D#0000ff> class=3D421064715-25102003> =
> ;
>
5-25102003>
>
A=
> llen A. Smith, Ph.D. Professor of Anatomy School of
> Graduate Medi=
> cal Sciences Barry University Miami Shores, FL
>
can anyone explain to me what lyses is....i have
> c=
> ome across this term several times while studying for my exam.
> UOTE>
The information transmitted is intended only for the
> =
> person or entity to which it is addressed and may contain confidential,
> a=
> nd/or privileged material. No confidentiality or privilege is waived or
> l=
> ost by any errant transmission. If you receive this message in error,
> ple=
> ase immediately delete it and all copies of it from your system and
> notif=
> y the sender. E-mail transmission cannot be guaranteed to be secure or
> er=
> ror-free as information could be intercepted, corrupted, lost,
> destroyed,=
> arrive late or incomplete, or contain viruses. Barry University -
> Mia=
> mi Shores, FL (http://www.barry.edu)