Re: [Histonet] Prefer fixative
More on the Prefer issue:
In a reference lab environment (like our lab), we have no choice in
what our client-colleagues use to fix their specimens, so working with
an open mind is not an option, but an obligation. I listed a few real
problems that our techs (who have decades of experience with generating
and validating antibodies) encounter with specimens fixed with Prefer.
I also hear similar complaints from experienced colleagues during
national advisory committee meetings.
If Prefer is used purely to produce adequate H&E results, it is a good
fixative. In terms of safety, I still look forward to long-term
follow-up results about its safety. I have no romance with formalin,
but any alternative fixative must be at least adequate for IHC and (the
increasingly used) FISH. The Mayo works closely with Vysis, and
Vysis/Abbott gives a long list of non-formalin fixatives (including
Prefer) that makes it difficult to get good results using FISH.
Finally, I am a pathologist, and I've learned a great deal from the
wonderful experience of histotechs and Medtechs through this forum on
technical issues, the same way I learn from techs in my workplace. On
the other hand, I occasionally offer advice to the Histonet only when I
feel it's useful to the group.
Hadi Yaziji, M.D.
On Wednesday, October 29, 2003, at 12:32 PM, Mary Bryhan wrote:
> I would like to set the record straight since I seemed to ruffle
> Hadi's feathers.
> Our institution does not perform FISH, but we do send them to Mayo
> Clinic in Rochester. We do our own immunohistochemistry and so far
> the only antibody that we have not been able to work up on Prefer
> fixed tissues is the TTF1.
> We have great results using Prefer fixative and I believe in this
> product. I believe that it does take an open mind, and a willingness
> on both staff and pathologists to work through the changes.
> Mary Bryhan HT (ASCP)
> Team Leader Anatomic Pathology
> Northern Michigan Hospital
> Petoskey, MI
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