Can you see the nucleus? If so, I don't understand the problem. Perhaps 
you are trying to stain the whole nucleated sperm one color and the 
whole anculeate sperm another? Otherwise, just stain for something found 
in a nucleus and not in the cytoplasm.

Geoff

Bruce Abaloz wrote:

>Hello Everyone,
>my name is Kathryn Mcnamara & I am a PhD student at the University of Melbourne, Australia. I am examining the sperm of moths. Moths have 2 types of sperm a nucleated (eupyrene) sperm and an anucleated (apyrene) sperm. 
>I was/AM hoping to find something that would only stain the nucleated sperm so that the two can be easily differentiated with the one stain. If you could help me it would be very much appreciated. THANKS in advance,
>
>Kathryn McNamara
>
>Department of Zoology
>University of Melbourne
>Victoria, Australia 3010
>
>
>  
>

-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
**********************************************




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