Re: [Histonet] (Histonet) Tunel Kit and Fixative

From:Jimmy Lao

I use the "ApopTag Fluorescein I situ Apoptosis
Detection Kit" from Serologicals Corporation
Maybe it has other company name: Intergen Company.
The one I used is anti-Dig conjugated rhodamine.
the disadvantage is that you need to distinguish the
deed cells from the autofluoresein cells like some
blood cell.  maybe selecting  fluoresein as the
conjugating fluorochrome is better.

--- Sharon E Willman  wrote:
> Hi,
> I was wondering if anyone  has information on Tunel.
>  What kit
> and fixative do you use?  Has anyone used Bouin's? 
> Any help you
> can give me would be most appreciated.
> Thanks,
> Sharon Willman
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