Re: [Histonet] Grocott's stain for fungi


Make sure you are using the correct chemical, it should be Hexamethylenetetramine (methenamine) (C6H12N4).  It should dissolve readily in H20.

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics

Kathleen Roberts <>
Sent by:

10/28/2003 09:51 AM

        To:        Histonet <>
        Subject:        [Histonet] Grocott's stain for fungi

Hi all-

I'm making up the various stock & working solutions for Grocott's stain,
and I am having a heck of a time getting the 3% methenamine to go into
solution.  (Not methenamine silver, just plain old 3% methenamine in
distilled water.)  I know that you have to acid-clean the glassware for
the methenamine silver & silver nitrate solutions, but do I have to do
it for 3% methenamine?  I tried heating it, but that did not make a bit
of difference. AFIP manual  has no other instructions other than "3 g
methenamine in 100 ml distilled water".

Is there a shelf life for methenamine?  The bottle we have is pretty
old, but I am not sure if that would be a factor.

I intend to try acid-cleaning a bottle for the 3% methenamine this
afternoon, but I wanted to see if anybody had any other suggestions to
try as well.

Thanks in advance-
Kathleen Roberts
Principal Lab Technician
Neurotoxicology Labs
Dept of Pharmacology & Toxicology
Ernest Mario School of Pharmacy
Rutgers University

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