RE: [Histonet] liquid nitrogen immersion

From:"Davis, Gareth"
[Histonet] liquid nitrogen immersion
Brandy,
When we have immersed tissue into Liquid Nitrogen, we have actually made our own cassettes with foil.  We just use regular foil and shape it around a cork or bottom of a small bottle (depending on size needed), leaving extra to fold over the frozen block - when finished freezing.  Put the freezing medium and tissue in foil cassette and immerse in LN until just about completely frozen, =small bubble in center results.  Then remove and store as in -80.
Hope this helps.
Gareth
 
Ms. Gareth B. Davis
Research Assistant II
Neuro-magnetics Division of
the Department of Neurology
Vanderbilt University Medical Center
615-936-3318
 
 
-----Original Message-----
From: Brandy Baca [mailto:BRBaca@salud.unm.edu]
Sent: Thu 10/30/2003 3:51 PM
To: histonet@lists.utsouthwestern.edu
Cc:
Subject: [Histonet] liquid nitrogen immersion

I am new to the listserve and have a question regarding immersion of
cassettes and vials in liquid nitrogen. I am going to be working with a
developing tissue bank and wondered if anyone knows what cassettes and
vials can withstand immersion in liquid nitrogen for quick-freezing. I
have spoke with Sakura and they have no evidence that their
uni-cassettes can withstand immersion. As for vials, many don't
reccomend immersion but some require a tube in order to accomodate this
process. If anyone can suggest a manufacturer and type, it would be
greatly appreciated. I would like to thank everyone in advance for their
responses.



Brandy Baca
Dept of Pathology
CRTC B-93
505.272.1127 phone
505.272.1370 fax

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