I had good collagen I staining with antibody from U of Iowa Hybridoma Bank 
with Pepsin digestion on formic acid decalcified rabbit bones.
Patsy



Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
HP-303-644-4538
HF-303-644-3377
hm email pruegg@msn.com
wk email pruegg@colobio.com

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----Original Message Follows----
From: "Mass Histology Service" 
To: "Rita Angel" ,
Subject: RE: [Histonet] collagen I staining
Date: Fri, 31 Oct 2003 14:50:30 -0500

We've had good luck with Calbiochem's anti-collagen I (Cat. # CP17L), 1:50
following pronase pre-treatment on rabbit bones.

Jim

____________________
James Staruk, HT(ASCP)
Mass Histology Service
A division of DVMLab, inc.
www.masshistology.com


-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu]On Behalf Of Rita Angel
Sent: Friday, October 31, 2003 2:23 PM
To: histonet@pathology.swmed.edu
Subject: [Histonet] collagen I staining


Hello Histonetters,

Someone in our lab has been trying to stain for collagen I antibody in
rabbit tissue. She's tried the antibody from several different companies
and has used different antigen retrievals, but has been unable to get any
positive staining. The only staining she's gotten is background. These are
paraffin sections. The companies she bought the antibodies from has not
been helpful. She's stained with Collagen type II & III and had no trouble
with these. Any suggestions would be helpful.

Thank you,
Rita Angel, HT
University of Cincinnati


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