I just get the feeling that if you have fixed the tissues properly then
there is 'relatively' little you can do to ruin it. I'm impressed that
you can buy all that just for the problem; try looking at your fixative.
Make sure its isotonic, the right pH and strength, and that you have
fixed it for long enough.

Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS
Tel: 01270 877625
Mob: 07830 196072 
Mobile E-Mail kemlorogerson@3mail.com                     
FAX & Answer Phone 0871 242 8094
E-mail Accounts:  
             kemlo@tiscali.co.uk or 
             kemlo1@btinternet.com 
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-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of Millicent
Bruce
Sent: 16 October 2003 16:47
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] burned artifact

For the past couple of years we have been getting a
burned artifact in our biopsies.  The pathologist have
said that on the slides the nucleus look smudged or
burned.  It is mostly on the small bxs, but we have
seen it on larger specimens also.  We have bought a
new tissue processor, and a new slide stainer thinking
that might be the problem.  We are still seeing the
artifact.  Is there anybody that might can tell us
what is causing the problem?  We have tried everything
we can think of.

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