RE: [Histonet] LMD ... IHC sections falling off

From:Deanne Catmull


Dear Victoria,

We always use Vectabond treated slides for immunohistochemistry. Sections
don't tend to float off. It's a simple procedure and good to use for pretty
much any stain. 

Deanne at La Trobe Bundoora
At 05:08 PM 10/23/03 -0500, mrl0627@mail.ecu.edu wrote:
>
> I sometimes lose sections (4 um thick, mounted on superfrost-plus) during
the counterstaining-clearing process although they survive immuno and
development in DAB.  Any thoughts of what I could be doing wrong?
>It's a heart breaker to lose them at the very end of the process.  I am in
a university lab and do everything by hand.  Thanks, Maureen at ECU
>-----Original Message-----
>From: Amos Brooks <amosbrooks@earthlink.net>
>To: histonet@lists.utsouthwestern.edu
>Sent: Mon, 20 Oct 2003 16:20:39 -0400
>Subject: [Histonet] LMD ... IHC sections falling off
>
>Victoria,
>         Firstly try thinner sections 10 um is a bit thick. Next you should 
>use some type of charged, silanized or APES slides. You can either silanize 
>your own slides or buy them from a vendor. Adhesives aren't necessarily 
>good for IHC as they can interfere with the reaction.
>Amos Brooks
>
>
>
>>Message: 5
>>From: victoria.hahn-stromberg@orebroll.se
>>To: histonet@lists.utsouthwestern.edu
>>Date: Mon, 20 Oct 2003 13:20:39 +0200
>>Subject: [Histonet] LMD
>>
>>Hi!
>>I=B4m having problems with my LMD slides.  I use 10um paraffin embedded
>>histological sections which I would like to stain immunohistochemically =
>>with
>>CAM, but the sections keep falling off, can anyone plesse help =
>>me??/Victoria
>>
>>Victoria Hahn-Str=F6mberg
>>MSc, PhD student
>>Kliniken f=F6r Patologi
>>Universitetssjukhuset =D6rebro
>>701 85 =D6rebro
>>tel:  019-6023644
>>faxnr:019-6021035
>
>
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Deanne Catmull	
Neuroimmunology Laboratory
Dept of Biochemistry
La Trobe University
Bundoora Vic 3086
Ph:9479-1155



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