RE: [Histonet] LMD ... IHC sections falling off

From:edmondsj

Hello,
Try incubating your slides for a longer period before deparaffinizing that 
may help with the sections washing off.  Hope this helps.
Joyce Edmonds
MUSC
Charleston, SC

--On Thursday, October 23, 2003 5:08 PM -0500 mrl0627@mail.ecu.edu wrote:

>
>  I sometimes lose sections (4 um thick, mounted on superfrost-plus)
> during the counterstaining-clearing process although they survive immuno
> and development in DAB.  Any thoughts of what I could be doing wrong?
> It's a heart breaker to lose them at the very end of the process.  I am
> in a university lab and do everything by hand.  Thanks, Maureen at ECU
> -----Original Message-----
> From: Amos Brooks <amosbrooks@earthlink.net>
> To: histonet@lists.utsouthwestern.edu
> Sent: Mon, 20 Oct 2003 16:20:39 -0400
> Subject: [Histonet] LMD ... IHC sections falling off
>
> Victoria,
>          Firstly try thinner sections 10 um is a bit thick. Next you
> should  use some type of charged, silanized or APES slides. You can
> either silanize  your own slides or buy them from a vendor. Adhesives
> aren't necessarily  good for IHC as they can interfere with the reaction.
> Amos Brooks
>
>
>
> >Message: 5
> >From: victoria.hahn-stromberg@orebroll.se
> >To: histonet@lists.utsouthwestern.edu
> >Date: Mon, 20 Oct 2003 13:20:39 +0200
> >Subject: [Histonet] LMD
> >
> >Hi!
> >I=B4m having problems with my LMD slides.  I use 10um paraffin
> embedded >histological sections which I would like to stain
> immunohistochemically = >with
> >CAM, but the sections keep falling off, can anyone plesse help =
> >me??/Victoria
> >
> >Victoria Hahn-Str=F6mberg
> >MSc, PhD student
> >Kliniken f=F6r Patologi
> >Universitetssjukhuset =D6rebro
> >701 85 =D6rebro
> >tel:  019-6023644
> >faxnr:019-6021035
>
>
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