I sometimes lose sections (4 um thick, mounted on superfrost-plus) during the counterstaining-clearing process although they survive immuno and development in DAB.  Any thoughts of what I could be doing wrong?
It's a heart breaker to lose them at the very end of the process.  I am in a university lab and do everything by hand.  Thanks, Maureen at ECU
-----Original Message-----
From: Amos Brooks <amosbrooks@earthlink.net>
To: histonet@lists.utsouthwestern.edu
Sent: Mon, 20 Oct 2003 16:20:39 -0400
Subject: [Histonet] LMD ... IHC sections falling off

Victoria,
         Firstly try thinner sections 10 um is a bit thick. Next you should 
use some type of charged, silanized or APES slides. You can either silanize 
your own slides or buy them from a vendor. Adhesives aren't necessarily 
good for IHC as they can interfere with the reaction.
Amos Brooks



>Message: 5
>From: victoria.hahn-stromberg@orebroll.se
>To: histonet@lists.utsouthwestern.edu
>Date: Mon, 20 Oct 2003 13:20:39 +0200
>Subject: [Histonet] LMD
>
>Hi!
>I=B4m having problems with my LMD slides.  I use 10um paraffin embedded
>histological sections which I would like to stain immunohistochemically =
>with
>CAM, but the sections keep falling off, can anyone plesse help =
>me??/Victoria
>
>Victoria Hahn-Str=F6mberg
>MSc, PhD student
>Kliniken f=F6r Patologi
>Universitetssjukhuset =D6rebro
>701 85 =D6rebro
>tel:  019-6023644
>faxnr:019-6021035


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