John,

I would demo every cryostat that I could as I find them somewhat user
specific.

I have not looked into things in a few years but do remember a cryostat I
think Bright that has a separate temperature control for the specimen stage
that is more finely tuned than most cryostats. I cutting fatty tissue I
think that would be very desirable. I have done some guinea pig and mink
skin in the past and think I would have loved to have that capacity.

Good Luck!  Hope you get what you need and want!

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317


-----Original Message-----
From: John McGinley [mailto:john.mcginley@colostate.edu]
Sent: Tuesday, October 28, 2003 5:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat purchase


Hi, 

Our research laboratory is looking at purchasing a cryostat in the near
future and we need a unit that will cut sections suitable for laser capture
microdisection (LCM). Most of our work revolves around rat mammary gland and
past attempts at cutting this very fatty tissue on an old IEC unit proved
frustrating to say the least. 

Our lab has been without a cryostat for a few years, so we don't know what
latest and greatest units are available. We're looking for good unit that
doesn't cost an arm and leg. Does anyone have any recommendations?

Also, is anyone using the Instrumedics CyroJane system for LCM? Does the
transfer tape interfere with RNA recovery? Any information would be greatly
appreciated.

Thanks,

John

----------------------------
John N. McGinley, HTL(ASCP)
Cancer Prevention Lab
Colorado State University
Email: john.mcginley@colostate.edu 


 


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