RE: [Histonet] Anyone have a favorite CD15 clone?

From:Tony Henwood

We compared two CD15 clones on several Hodgkin's disease cases and found
LeuM1 the best. Staining overall was improved with both antibodies if
overnight incubation (4oC) was used.

Remember that most CD15 antibodies are IgM (not IgG), and therefore bigger.
Also the detection system may not be all that efficient for IgM antibodies.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Drew Sally A. []
Sent: Wednesday, 22 October 2003 6:39
To: Histonet
Subject: [Histonet] Anyone have a favorite CD15 clone?

We are just wondering if not all clones of CD15 are equal, and that maybe
out there has tested more than a couple different CD15's?  We have
who periodically ask us to repeat our stain because they expect to see more
RS cells staining than they do.  We currently run our antibodies on Ventana 
immunostainers if anyone feels that makes a difference.  
All constructive opinions are welcome.
 Thank you!

Sally Ann Drew-MT(ASCP)
Univ. of Wisconsin Hospital & Clinics
IHC/ISH Laboratory 
600 Highland Ave. VAH-DM223
Madison, WI 53792-2472

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