[Histonet] rhodamine auto-fluor. problem

From:"Bonnie P Whitaker"

Hi All!
I trust everyone that went to Louisville had a wonderful time and learned a lot!!
I have a question for you guys:  A researcher here in OB/GYN is doing rhodamine-labeled fluorescent work (he didn't say what antibody) on mouse fallopian tube... he thought he was having a staining problem, but has determined that his tissue is auto-fluorescing.  The tissue is frozen in OCT and fixed in acetone/methanol.  What can he do to quench this?

Bonnie Whitaker
University of Texas Medical School at Houston
6431 Fannin Street
MSB 2.231
Houston, Texas  77030
Phone 713.500.6792
Fax 713.500.0733


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