[Histonet] mouse brains- sucrose cryoprotection- sectioning

From:Yogeshwar Kalkonde

Hi Histonetters-
i am trying do Nissl staining on mice brain sections. Mice brains were fixed 
in 4%PFA and cryoprotected by overnight treatment with 30% sucrose. Brains 
were then embedded in OCT and frozen.
while trying to section the the tissues in a cryostat (@8 microns thickness) 
the sections shrink, distorting the tissue architecture. 
any clue what might be going wrong?
thanks in advance...

Yogeshwar V. Kalkonde, MD.
Post Doctoral Fellow,
Department of Medicine (Infectious Diseases)
University Of Texas Health Sciences Center at San Antonio, 
7703 Floyd Curl Drive, 5.084R, Mail Code 7870,
San Antonio, TX 78229-3900
Ph: 210-567-0385 (lab)

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