Hi, 
I'm having troubles finding my b-gal-expressing plasmid in the lung
tissue. I do 5-10 um frozen sections of murine lungs, fix them for 10
minutes in 0.2% glutaraldehyde, wash twice in a detergent wash, and
incubate overnight in regular lacZ staining solution. 

I am sure my lacZ is there, because i can see it when I stain my lungs
macroscopically.

Thanks 

Tom Kuzniar



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