[Histonet] When to use tap water?


Hello, all:
The prof for my graduate course in histology insisted that tap water be used for rinsing slides during certain staining procedures (eg. H&E)although he did not give a specific reason why.  
When no "flavor" of water is specified in a procedure, I generally use MilliQ or distilled deionized water.  Is this the safest route to take or should one use tap if nothing but "water" is listed?
Thanks for the attention.  Maureen, MS candidate at ECU.

-----Original Message-----
From: "Houston, Ronnie" <Ronnie_Houston@bshsi.com>
To: "'Morken, Tim - Labvision'" <tpmorken@labvision.com>,	histonet@lists.utsouthwestern.edu
Sent: Tue, 28 Oct 2003 13:05:45 -0500
Subject: RE: [Histonet] Dionized vs distilled water

What quality of water is recommended/regulated for anatomic and clinical
pathology labs?
Ronnie Houston 
Regional Histology Operations Manager 
Bon Secours HealthPartners Laboratories 
5801 Bremo Road 
Richmond, VA 23226 
(804) 287 7972 
-----Original Message-----
From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com]
Sent: Tuesday, October 28, 2003 12:53 PM
To: 'JCarpenter764@aol.com'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dionized vs distilled water

Distilled water is classically produced by heating water to evaporation
and subsequent condensing on a cold surface. In the process most
impurities are either evaporated off ahead of the water (in the case of
most organics), or left behind (in the case of minerals). The water is
also effectively deionized because the salts are left behind. It is
fairly pure water. To get very pure water it needs to be re-distilled
several times.
Deionized water is classically passed through a salt bed or ionized
resin bed that captures the mineral ions (ie, a "water softener"). The
water is not necessarily pure, however, especially in regards to organic
chemicals. Reverse osmosis is also used now days to deionize water.
High quality water systems these days are some combination of filters,
distillation, deionizing resins and reverse osmosis. 
Tim Morken
-----Original Message-----
From: JCarpenter764@aol.com [mailto:JCarpenter764@aol.com] 
Sent: Tuesday, October 28, 2003 7:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
while studying for my exam on the different fixatives and there
ingredients....i have noticed that some call for distilled water and
some use the term deionized water.  Is there a difference?



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