[Histonet] Re: Histonet digest, Vol 1 #103 - 18 msgs
Noelle,
We've been working on this for a long time with constant
inconsistant results. We've used many vendors antibodies and none have
performed properly. (BTW, we run a multitude of other antibodies and they
all work fine so the problem is the antibody not the rest of the process)
If you're doomed to follow the VEGF path I highly advise that you
run negative controls on it. You're bound to notice similarities. If you
read about VEGF in literature do so with a highly skeptical mind.
Good luck,
Amos Brooks
At 01:00 PM 10/27/03, you wrote:
>Hi all!
>
>Does anyone have a decent protocol for VEGF, preferably on rat FFPE? =
>
>Thank you!!
>
>Noelle Linke, BS, HTL(ASCP)
>Allergan, Inc
>2525 Dupont Drive RD-2A
>Irvine, CA 92612
>714-246-5568
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>