Cowen T, Haven AJ, Burnstock G.  Pontamine sky blue: a counterstain for
background autofluorescence in
fluorescence and immunofluorescence histochemistry.  Histochemistry.
1985;82(3):205-8.

The stain pontamine sky blue (PSB) has been shown to reduce background
autofluorescence in catecholamine fluorescence and immunofluorescence
histochemical preparations. Using PSB as a counterstain on whole-mount
stretch
preparations of human mesenteric blood vessels, a medium dense noradrenergic
nerve plexus is clearly revealed, which previously had been only partially
visible because of background autofluorescence. Image analysis of nerve
densities in whole-mount stretch preparations of guinea-pig arteries
containing
noradrenergic, substance P-, and vasoactive intestinal polypeptide
(VIP)-positive nerve plexuses shows that PSB staining does not alter the
specific neuronal fluorescence and that it improves image definition.

If you subscribe to Loansome Doc, you can order a pdf copy for a price.

Gary Gill


-----Original Message-----
From: Sven Terclavers [mailto:Sven.Terclavers@med.kuleuven.ac.be]
Sent: Tuesday, October 28, 2003 11:55 AM
To: Microscopy@msa.microscopy.com; histonet@lists.utsouthwestern.edu
Subject: [Microscopy] Looking for old article




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Hi all,

I'm looking for a pdf or word-document of the following qrticle. Can anyone
help me out?
Thanks!

Histochemistry. 1985;82(3):205-8.
Cowen T, Haven AJ, Burnstock G.
Pontamine sky blue: a counterstain for background autofluorescence in
fluorescence and immunofluorescence histochemistry.

Sven Terclavers


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