Hello histonetters,
I have an Olympus BH-2 microscope fitted with a real nice PM10AD Olympus
film camera. I have been real happy with the set up as I take a lot of
publication photos. My problem is digital is now the way to go. I
borrowed a Coolpix MDC lens adapter with a coolpix and Olympus C-5050
camera. I not very pleased with the results. Does anyone have a
recommendation for me.
Best
Gordon Grant
-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Saturday, October 25, 2003 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet digest, Vol 1 #101 - 12 msgs
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When replying, please edit your Subject line so it is more specific
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Today's Topics:
1. Re: Histonet digest, Vol 1 #100 - 15 msgs (amosbrooks)
2. Re: CD 25 (Dana Settembre)
3. Re: Moth Sperm. (Geoff McAuliffe)
4. can someone please explain lyses (JCarpenter764@aol.com)
5. (no subject) (Wright, Barbara (SPRI 2))
6. CD25 (Dawson, Glen)
7. rhodamine auto-fluor. problem (Bonnie P Whitaker)
8. (no subject) (JCarpenter764@aol.com)
9. RE: (no subject) (Barry R Rittman)
10. Reality Check (Joe Nocito)
11. Re: CD23 and BCL-6 on B5 fixed tissue (Hadi Yaziji)
12. RE: (no subject) (Smith, Allen)
--__--__--
Message: 1
Date: Fri, 24 Oct 2003 10:46:00 -0700 (PDT)
From: amosbrooks
Reply-To: amosbrooks@earthlink.net
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet digest, Vol 1 #100 - 15 msgs
Ronnie,
We use Novocastra's CD25 (NCL CD25 305) at 1:100. We pretreat with
hot
citrate buffer (HIER) and detect with Envision.
Amos Brooks
From: "Houston, Ronnie"
To: "Histonet (E-mail)"
Date: Thu, 23 Oct 2003 12:58:03 -0400
Subject: [Histonet] CD 25
Is anyone using CD25 on formalin-fixed, paraffin-
embedded human tissue? What clone and source are available?
Thanks
Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972
ronnie_houston@bshsi.com
--__--__--
Message: 2
Date: Fri, 24 Oct 2003 14:09:07 -0400
From: Dana Settembre
To: Ronnie_Houston@bshsi.com, histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] CD 25
Hello Ronnie,
I use CD25 (Interleukin-2 Receptor) from Novocastra Laboratories which
is distributed by Vector Laboratories in Burlingame, CA.
I use in on FFPE human tissue. Their clone is 4C9. Their catalog
number is NCL-CD25-305.
Their spec sheet recommends a tonsil for a positive control.
I use it at 1:50 with Dako's Target Retrieval Solution.
Good Luck.
Dana Settembre
University Hospital-UMDNJ
Newark, NJ
>>> "Houston, Ronnie" 10/23/2003 9:58:03 AM
>>>
Is anyone using CD25 on formalin-fixed, paraffin-
embedded human tissue? What clone and source are available?
Thanks
Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond, VA 23226
(804) 287 7972
ronnie_houston@bshsi.com
________________________________________________________________________
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--__--__--
Message: 3
Date: Fri, 24 Oct 2003 14:14:46 -0400
From: Geoff McAuliffe
To: Bruce Abaloz
Cc: HistoNet ,
histonet-admin@lists.utsouthwestern.edu
Subject: Re: [Histonet] Moth Sperm.
Can you see the nucleus? If so, I don't understand the problem. Perhaps
you are trying to stain the whole nucleated sperm one color and the
whole anculeate sperm another? Otherwise, just stain for something found
in a nucleus and not in the cytoplasm.
Geoff
Bruce Abaloz wrote:
>Hello Everyone,
>my name is Kathryn Mcnamara & I am a PhD student at the University of
Melbourne, Australia. I am examining the sperm of moths. Moths have 2
types of sperm a nucleated (eupyrene) sperm and an anucleated (apyrene)
sperm.
>I was/AM hoping to find something that would only stain the nucleated
sperm so that the two can be easily differentiated with the one stain.
If you could help me it would be very much appreciated. THANKS in
advance,
>
>Kathryn McNamara
>
>Department of Zoology
>University of Melbourne
>Victoria, Australia 3010
>
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
**********************************************
--__--__--
Message: 4
From: JCarpenter764@aol.com
Date: Fri, 24 Oct 2003 14:23:51 EDT
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] can someone please explain lyses
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Message: 5
From: "Wright, Barbara (SPRI 2)"
To: "'histonet@pathology.swmed.edu'"
Date: Fri, 24 Oct 2003 15:20:12 -0400
Subject: [Histonet] (no subject)
This message is in MIME format. Since your mail reader does not
understand
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------_=_NextPart_001_01C39A63.D8B02C50
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To all my well versed colleagues --
I am in the pursuit of slide labels that are sold in 8.5X11 sheets that
can
be used in a laser printer. (I haven't decided to use word, excel or
file
maker pro formats to generate the labels). Any help would be greatly
appreciated.
Thanks,
Barb
Barbara Wright
Scientist II
Exp. Pathology & Pharmacology
DNAX
901 California Avenue
Palo Alto, CA 94304-1104
barbara.wright2@dnax.org
*********************************************************************
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-- Please immediately and permanently delete.
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To all my well versed colleagues
--
I am in the pursuit of slide labels
that a=
re sold in 8.5X11 sheets that can be used in a laser printer. (I
have=
n't decided to use word, excel or file maker pro formats to generate the
la=
bels). Any help would be greatly appreciated.
Thanks,
Barb
Barbara
Wright Scientist
II Exp. Pathology
&am=
p; Pharmacology DNAX 901 California
Ave=
nue Palo Alto, CA
9430=
4-1104 barbara.wright2@dn=
ax.org
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If =
you are not the intended recipient, disclosure, copying, use or
distributio=
n of the information included in this message is prohibited -- Please
immed=
iately and permanently delete.
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--__--__--
Message: 6
From: "Dawson, Glen"
To: histonet@lists.utsouthwestern.edu
Date: Fri, 24 Oct 2003 15:01:36 -0500
Subject: [Histonet] CD25
Ronnie,
I use CD25 (clone Tu69) from Novocastra. Cat.# NCL-CD25-305. It is for
FFPE tissues.
Good Luck,
Glen Dawson
--__--__--
Message: 7
Reply-To:
From: "Bonnie P Whitaker"
To: "histonet"
Date: Fri, 24 Oct 2003 16:59:39 -0500
Subject: [Histonet] rhodamine auto-fluor. problem
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Hi All!
I trust everyone that went to Louisville had a wonderful time and
learned a
lot!!
I have a question for you guys: A researcher here in OB/GYN is doing
rhodamine-labeled fluorescent work (he didn't say what antibody) on
mouse
fallopian tube... he thought he was having a staining problem, but has
determined that his tissue is auto-fluorescing. The tissue is frozen in
OCT
and fixed in acetone/methanol. What can he do to quench this?
Thanks,
Bonnie Whitaker
University of Texas Medical School at Houston
6431 Fannin Street
MSB 2.231
Houston, Texas 77030
Phone 713.500.6792
Fax 713.500.0733
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Hi All! =
I trust everyone =
that went to=20
Louisville had a wonderful time and learned a lot!!
I have a question =
for you=20
guys: A researcher here in OB/GYN is doing rhodamine-labeled =
fluorescent=20
work (he didn't say what antibody) on mouse fallopian tube... he thought
=
he was=20
having a staining problem, but has determined that his tissue is=20
auto-fluorescing. The tissue is frozen in OCT and fixed in=20
acetone/methanol. What can he do to quench =
this?
Thanks,
Bonnie Whitaker University of Texas
=
Medical=20
School at Houston 6431 Fannin Street MSB 2.231 Houston, =
Texas =20
77030 Phone 713.500.6792 Fax 713.500.0733
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--__--__--
Message: 8
From: JCarpenter764@aol.com
Date: Fri, 24 Oct 2003 18:29:08 EDT
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
-------------------------------1067034548
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can anyone explain to me what lyses is....i have come across this term
several times while studying for my exam.
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can anyone explain to me what lyses is....i have come across this
term se=
veral times while studying for my exam.
-------------------------------1067034548--
--__--__--
Message: 9
Date: Fri, 24 Oct 2003 18:40:14 -0500
From: "Barry R Rittman"
To: ,
"histonet"
Subject: RE: [Histonet] (no subject)
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bWVzIHdoaWxlIHN0dWR5aW5nIGZvciBteSBleGFtLg0KDQo=
--__--__--
Message: 10
From: "Joe Nocito"
To: "Histonet"
Date: Fri, 24 Oct 2003 21:13:33 -0700
Subject: [Histonet] Reality Check
I want to wish everyone back from the NSH meeting. Hope you had a
good
time. Maybe I can attend next year.
I have this issue that just won't go away and I would like your
opinions.
In the last three years, I've purchased 4 TBS waterbaths, two of
which
the heating element went bad. One was three years old, the other about
two
years. Now, I realize that TBS has a one year warranty on their
equipment,
but shouldn't waterbaths last longer than 3 years? I paid over $1000 per
waterbath and they want $500 a piece to repair them. I have seen
Boekel,
Lab-Line and Fisher waterbaths that were older than me (we won't go
there
ok?) Which is to say that I'm much older than 3 years.
Has anyone else come across this problem? I'm just waiting for the
other 2 waterbaths to breakdown. I could see maybe one waterbath, but
2,
with the same problem. I think the odds are just too high. Am I like
in
outer space or does this bother anyone else? Maybe it's me. I don't
know.
Oh, as a last comment, we do not suffer from severe power surges either,
just in case someone would ask. Take care and thanks again for your
input.
Joe Nocito BS, HT (ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, Texas
--__--__--
Message: 11
Date: Fri, 24 Oct 2003 21:12:16 -0700
Cc:
To: "Doug Geddes"
From: Hadi Yaziji
Subject: Re: [Histonet] CD23 and BCL-6 on B5 fixed tissue
Dear Doug,
B5 fixative destroys the signal of many lymphoid markers. Please advise
your pathologists that there's no reason to use it on lymphoid cases.
Best regards,
Hadi Yaziji, M.D.
PhenoPath Laboratories
On Friday, October 24, 2003, at 08:47 AM, Doug Geddes wrote:
> Looking for any information on performance of CD23 and BCL-6
antibodies
> on B5 fixed tissue, re - antibody suppliers, incubation times, antigen
> retreival(?). Thanks so much in advance.
>
> Doug Geddes BSc, MLT
> LHSC, London ON, Canada
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--__--__--
Message: 12
Date: Sat, 25 Oct 2003 11:59:21 -0400
From: "Smith, Allen"
To:
Cc:
Subject: RE: [Histonet] (no subject)
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Lyses is the the third person singular of the verb "to lyse". It means
"=
to
cause the breakdown of." E.g., "Complement lyses bacteria," or, "Dia=
stase
lyses glycogen."
Once in a blue moon, lyses is used as the plur=
al of lysis, which is Latin
for dissolving. In English, lysis usually m=
eans breakdown. In chemistry,
it means the splitting of a compound into=
two parts. In biology, it means
the death of a cell and the disappeara=
nce of its parts.
Allen A. Smith, Ph.D.
Professor of Anatomy
School=
of Graduate Medical Sciences
Barry University
Miami Shores, FL
--=
---Original Message-----
From: JCarpenter764@aol.com [mailto:JCarpenter=
764@aol.com]
Sent: Friday, October 24, 2003 6:29 PM
To: histonet@lis=
ts.utsouthwestern.edu
Subject: [Histonet] (no subject)
can anyo=
ne explain to me what lyses is....i have come across this
term several t=
imes while studying for my exam.
=
The information transmitted is intended only for the =
person or entity to which it is addressed and may contain confidential,
a=
nd/or privileged material. No confidentiality or privilege is waived or
l=
ost by any errant transmission. If you receive this message in error,
ple=
ase immediately delete it and all copies of it from your system and
notif=
y the sender. E-mail transmission cannot be guaranteed to be secure or
e=
rror-free as information could be intercepted, corrupted, lost,
destroyed=
, arrive late or incomplete, or contain viruses.
Barry University - Miam=
i Shores, FL (http://www.barry.edu)
------_=_NextPart_001_01C39B10.F4706942
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Content-Transfer-Encoding: quoted-printable
Message
Lyses is the the
third person singular of th=
e verb "to lyse". It means "to cause the
breakdown of." E.g=
., "Complement lyses bacteria," or, "Diastase lyses
glycogen."
Once in a
blue moon, lyses is used as the
plural of lysis, which is Latin =
for dissolving. In English, lysis usually
means breakdown. =
In chemistry, it means the splitting of a compound into
two parts. =
; In biology, it means the death of a cell and the disappearance
of its=
parts.
=
;
A=
llen A. Smith, Ph.D. Professor of Anatomy School of
Graduate Medi=
cal Sciences Barry University Miami Shores, FL
=
-----Original Message----- Fr=
om:
JCarpenter764@aol.com [mailto:JCarpenter764@aol.com] S=
ent: Friday,
October 24, 2003 6:29 PM To:
histonet@=
lists.utsouthwestern.edu Subject: [Histonet] (no
subject)
can anyone explain to me what lyses is....i have
c=
ome across this term several times while studying for my exam.
The information transmitted is intended only for the
=
person or entity to which it is addressed and may contain confidential,
a=
nd/or privileged material. No confidentiality or privilege is waived or
l=
ost by any errant transmission. If you receive this message in error,
ple=
ase immediately delete it and all copies of it from your system and
notif=
y the sender. E-mail transmission cannot be guaranteed to be secure or
er=
ror-free as information could be intercepted, corrupted, lost,
destroyed,=
arrive late or incomplete, or contain viruses. Barry University -
Mia=
mi Shores, FL (http://www.barry.edu)