[Histonet] Not all tissue staining...

From:"Bauer, Karen"

Hello to all,
There are times when a stained IP slide will have only half of the tissue
staining positively and not other parts of the tissue, but has the same
cellularity of the part that is staining. (Did that make sense?)  Same
tissue cells, but some stain and some do not.  When the Pathologist asks why
this happens, I can only come up with possible vortex mixing problems or
that the paraffin did not come out completely.  We've recently switched to
the BenchMark and this has only happened a few times.  I've checked the
vortex mixers, and all of the heating pads are working properly.  

Does anyone have any suggestions?

Thanks in advance.

Karen L. Bauer HT(ASCP)
Histology Department
Luther Hospital

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