[Histonet] NADPH diaphorase
I'm going to try do some NADPH diaphorase on brain sections. I
assume you can't do the technique on formalin fixed, paraffin
embedded tissue, but I found a protocol in Current Protocols in
Neuroscience which says you can do the technique on tissue fixed in
paraformaldehyde which has been put on a slide prior to staining.
Does anyone know if this would work (if not should I use floating
sections?) and am I correct in assuming that paraffin embedded tissue
won't work?
Thanks,
Sharon
--
Sharon Cooperman
NIH, NICHD, CBMB 301.435-7735
Building 18T, room 101 301.402-0078 fax
Bethesda, MD 20892
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