[Histonet] NADPH diaphorase

From:Sharon Cooperman

I'm going to try do some NADPH diaphorase on brain sections.  I 
assume you can't do the technique on formalin fixed, paraffin 
embedded tissue, but I found a protocol in Current Protocols in 
Neuroscience which says you can do the technique on tissue fixed in 
paraformaldehyde which has been put on a slide prior to staining. 
Does anyone know if this would work (if not should I use floating 
sections?) and am I correct in assuming that paraffin embedded tissue 
won't work?

Thanks,
Sharon
-- 
Sharon Cooperman        	     
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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