[Histonet] Grocott's stain for fungi
I'm making up the various stock & working solutions for Grocott's stain,
and I am having a heck of a time getting the 3% methenamine to go into
solution. (Not methenamine silver, just plain old 3% methenamine in
distilled water.) I know that you have to acid-clean the glassware for
the methenamine silver & silver nitrate solutions, but do I have to do
it for 3% methenamine? I tried heating it, but that did not make a bit
of difference. AFIP manual has no other instructions other than "3 g
methenamine in 100 ml distilled water".
Is there a shelf life for methenamine? The bottle we have is pretty
old, but I am not sure if that would be a factor.
I intend to try acid-cleaning a bottle for the 3% methenamine this
afternoon, but I wanted to see if anybody had any other suggestions to
try as well.
Thanks in advance-
Principal Lab Technician
Dept of Pharmacology & Toxicology
Ernest Mario School of Pharmacy
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