[Histonet] Embryo sticking in InsituPro Run

From:Jimmy Lao

Hi
Any one can help preventing embryo sticking to the
Column during InsituPro run? InsituPro is automated
machine for whole mount embryo in situ hybridization. 
It did not happen when I used fresh column. But it did
in the 2nd time using column.  I guess there are
something staying in the column after first use, even
I treated them with H2O2 overnight and 4%PFA cleaning
program treatment.  How can I clean used column?
Thanks!
Jimmy

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