Fw: [Histonet] Sirius Red Technique.

From:"Bryan Llewellyn"

----- Original Message -----
From: Bryan Llewellyn
To: Ian Montgomery
Sent: Wednesday, October 29, 2003 9:48 AM
Subject: Re: [Histonet] Sirius Red Technique.

It very slowly deteriorates over time, but it can be compensated for by extending staining time.  I have used solutions a year old with 2 hours staining, but that was stretching it a bit.  Six months would be OK.  So one hour fresh, 2 hours at six months.  Incidentally, it is better if no more than 2 mL salt solution is added.  The absolute minimum salt is the critical step.
Bryan Llewellyn
----- Original Message -----
From: Ian Montgomery
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, October 29, 2003 2:46 AM
Subject: [Histonet] Sirius Red Technique.

        Quick question and the man himself may be able to answer. Sirius Red Technique (Llewellyn, 1970), what is the shelf life of the prepared stain?

Dr. Ian Montgomery,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery@bio.gla.ac.uk

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