Fw: [Histonet] Sirius Red Technique.
----- Original Message -----
Sent: Wednesday, October 29, 2003 9:48 AM
Subject: Re: [Histonet] Sirius Red Technique.
It very slowly deteriorates over time, but it can
be compensated for by extending staining time. I have used solutions a
year old with 2 hours staining, but that was stretching it a bit. Six
months would be OK. So one hour fresh, 2 hours at six months.
Incidentally, it is better if no more than 2 mL salt solution is added.
The absolute minimum salt is the critical step.
Bryan Llewellyn
----- Original Message -----
Sent: Wednesday, October 29, 2003 2:46
AM
Subject: [Histonet] Sirius Red
Technique.
Quick
question and the man himself may be able to answer. Sirius Red Technique
(Llewellyn, 1970), what is the shelf life of the prepared
stain?
Ian.
Dr. Ian
Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of
Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12
8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625
702883
e-mail: ian.montgomery@bio.gla.ac.uk
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