Re: [Histonet] STATIC AT THE MICROTOME
Dry atmospheric conditions could be the culprit, also the clothes that you
wear. Synthetic fabrics can cause real headaches.
For dry conditions, some folks gently breathe on the block before sectioning;
others dip their thumb in water and then gently rub their thumb on the block
surface. Some folks use a small humidifier in the vicinity of the microtome.
Others burn a small alcohol lamp somewhere nearby. Anything to increase the
humidity in the vicinity of the block is worth a try.
There is an interesting discussion in the Histonet Archives about this
problem. The discussion thread mainly concerns static electricity in cryostats, and
of course breathing on a frozen specimen is a no-no! But look for messages
concerning "Breathers" and "Huffers" (the posters' choice of words, not mine!)
These messages talk about static electricity and paraffin sections.
For the Histonet community that might not know about it, the Histonet search
engine is fantastic. I use it a lot. Here's the link:
Click here: Histosearch: The Histology
Just click on the box and select "Histonet Archives" as the database to
search, enter your search words, and you'll get more info than you thought was out
there. I used "static electricity" as the search words (without the quote
marks). You would probably get more pertinent results by searching for
"breathers" and "huffers."
Good luck, hope this helps.
Leica Exclusive Regional Dealer
Desert Southwest Region, USA
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