According to Carson, the demonstration of
chromaffin granules canot be demonstrated after formalin fixation.
(Histotechnology: A Self-Instructional Text,
page 6-7)
Jennifer MacDonald
----- Original Message -----
Sent: Thursday, October 09, 2003 2:14
PM
Subject: Re: [Histonet] Need silver stain
for granules in adrenals
I don't think you can get a good chromaffin reaction on
material already fixed with formalin. Fontana-Masson should be fine. John
Kiernan published a chromate-dichromate fix for chromaffin tissues, it may be
in his book, along with other recommendations.
Geoff
Nick Kirk
wrote:
What you are trying to demonstrate are Chromaffin
cells of the adrenal glands so a Masson-Fontana should work, if not try a
Schmorl or carry out the Chromaffin reaction
If
you use Iodate oxidation you can distinguish between Adrenaline and
noradrenalin fairly easily as iodates oxidise noradrenaline much quicker
than they do adrenaline.
Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England
Hi,
Could anyone suggest a stain for noradrenalin and adrenalin granules
in paraffin embedded adrenals. We were asked to do a gremilius stain
and it did not stain the granules. Are these slides lost or can we
re stain them? We usually do a recut of the block, but it is asked
if we could use the same tissue slide. The stain actually worked, but not
for the granules.
From what we read a Fontana-masson should work. There probably
is a better stain our there for this specific target. Can anyone
with some experience with staining these granules share their staining
protocol.
At this time the request is not for
immuno staining.
Thanks for your help.
Nancy Maronto
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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