Re: [Histonet] Need silver stain for granules in adrenals

From:"Jennifer MacDonald"

According to Carson, the demonstration of chromaffin granules canot be demonstrated after formalin fixation.
(Histotechnology:  A Self-Instructional Text, page 6-7)
Jennifer MacDonald
----- Original Message -----
From: Geoff McAuliffe
To: Nick Kirk
Cc: Histonet
Sent: Thursday, October 09, 2003 2:14 PM
Subject: Re: [Histonet] Need silver stain for granules in adrenals

I don't think you can get a good chromaffin reaction on material already fixed with formalin. Fontana-Masson should be fine. John Kiernan published a chromate-dichromate fix for chromaffin tissues, it may be in his book, along with other recommendations.


Nick Kirk wrote:
What you are trying to demonstrate are Chromaffin cells of the adrenal glands so a Masson-Fontana should work, if not try a Schmorl or carry out the Chromaffin reaction
If you use Iodate oxidation you can distinguish between Adrenaline and noradrenalin fairly easily as iodates oxidise noradrenaline much quicker than they do adrenaline.
Nick Kirk
Hinchingbrooke Hospital
-----Original Message-----
From: []On Behalf Of Nancy Maronto
Sent: 09 October 2003 16:05
Subject: [Histonet] Need silver stain for granules in adrenals

Could anyone suggest a stain for noradrenalin and adrenalin granules in paraffin embedded adrenals.  We were asked to do a gremilius stain and it did not stain the granules.  Are these slides lost or can we re stain them?  We usually do a recut of the block, but it is asked if we could use the same tissue slide. The stain actually worked, but not for the granules.
From what we read a Fontana-masson should work.  There probably is a better stain our there for this specific target.  Can anyone with some experience with staining these granules share their staining protocol.
At this time the request is not for immuno staining. 
Thanks for your help.
Nancy Maronto

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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