What you are trying to demonstrate are
Chromaffin cells of the adrenal glands so a Masson-Fontana should work,
if not try a Schmorl or carry out the Chromaffin reaction
If you use Iodate oxidation you can
distinguish between Adrenaline and noradrenalin fairly easily as
iodates oxidise noradrenaline much quicker than they do adrenaline.
Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England
Hi,
Could anyone suggest a stain for noradrenalin and adrenalin
granules in paraffin embedded adrenals. We were asked to do a
gremilius stain and it did not stain the granules. Are these slides
lost or can we re stain them? We usually do a recut of the block, but
it is asked if we could use the same tissue slide. The stain actually
worked, but not for the granules.
From what we read a Fontana-masson should work. There
probably is a better stain our there for this specific target. Can
anyone with some experience with staining these granules share their
staining protocol.
At this time the request is not for immuno staining.
Thanks for your help.
Nancy Maronto