Re: [Histonet] Keeping tissues on membrane slides
Hi, membrane slides are like plastic wrap over a glass slide, hand glued or
you can buy them in a hard plastic cut-out slide with the plastic membrane
sealed into it. I'm not sure you can charge the membrane but I don't lay it on
the water bath. It seems that the 12 microns that the researcher wanted it cut
at was too thick. I have since found out it should be cut at 6 microns for
laser cutting and DNA studies. But I will keep your info in mind when I cut with
charged slides. Thanks for the tip.
Lil Muolo
Immuno. Lab
Cancer Institute of New Jersey
>>> Margaret Horne
<mhorne@upei.ca> 10/1/03 10:45:20 AM >>>
I don't know about membrane slides , but I had a lot of problems
with
tissue floating off during IHC. Then someone pointed out that I
was using
charged slides but laying them out on the side of the
water bath. The water
bath is grounded and therefore I may have
been losing the charge on the
slide and it's the charge that keeps
ithe tissue on. Stopped touching the
slides to the water bath and
stopped losing tissue.What can I say... it
worked for me.
Membrane slides may be very different. I don't know.
Good
luck,
Margaret
Margaret Horne ,
Histology Teaching Assistant,
Dept. of
B.SC.,
Atlantic Veterinary College, U.P.E.I.,
550 University Ave.,
Charlottetown,
P.E.I., C1A 4P3
Canada
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