Re: [Histonet] Jones/PAS

From:"Bryan Hewlett"

It would be pointless to perform a PAS and a Jones silver together as a combination since they both demonstrate the same elements.
Jones silver method for basement membranes, uses the methenamine silver as an aldehyde detection system in place of the Schiff reagent used in PAS.
The reason for this is to provide a more intense and contrasty demonstration of the basement membrane in thin sections of kidney. A PAS stain tends to be too weak in 1-2 micrometer sections.
----- Original Message -----
From: Turner, Scott
To: HistoNet Server (
Sent: Monday, October 13, 2003 7:57 PM
Subject: [Histonet] Jones/PAS

A colleague of mine needs to do a Jones' silver stain in kidney in combination with PAS.  Does anyone have a protocol for doing these two together?

Scott Turner
DNAX Research Institute
Palo Alto, CA

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