Re: [Histonet] IHC QC's

From:"Patsy Ruegg"

IHC QC will be a topic discussed at the IHC Resource Group committee meeting 
during the NSH S/C in Louisville.  The committee meeting will be on Saturday 
at 5:30 PM.  I invite all those attending the NSH S/C with an interest in 
IHC to attend this meeting.
Best regards,

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
hm email
wk email

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----Original Message Follows----
From: Patti Loykasek 
To: "Horn, Hazel V" ,histonet 

Subject: Re: [Histonet] IHC QC's
Date: Mon, 13 Oct 2003 14:18:00 -0700

There was in fact, a post from Nick Kirk on running a positive control with
each case. I do realize the CAP requirements and am familiar with the

Patti Loykasek
Phenopath Laboratories
Seattle, WA
 > I don't think anyone said a POSTIVE control should be run with each 
 > We were talking about negative controls, I believe.
 > I just copied and pasted from the latest CAP survey in another email.
 > Hazel Horn, HT/HTL (ASCP)
 > Histology Supervisor
 > Arkansas Children's Hospital
 > Phone - 501.364.4240
 > Fax - 501.364.3912
 >> -----Original Message-----
 >> From: Patti Loykasek []
 >> Sent: Monday, October 13, 2003 10:52 AM
 >> To: histonet
 >> Subject: [Histonet] IHC QC's
 >> I'm glad that everyone is so concerned with both negative and positive 
 >> controls. There is certainly more than one side to this issue. I will 
 >> that I don't think a positive QC on every slide is absolutely necessary, 
 >> many reasons. If the QC is rare & precious, then it is a waste of 
 >> As is running a negative control for every possible technique 
permutation on
 >> small amounts of tumor. I would rather have slides with tumor left for
 >> additional studies than have wasted tumor sections on 4-6 negative 
 >> You can always evaluate non-specific staining on slides that have had an
 >> antibody applied & that are negative with that antibody. The CAP is 
 >> that positive controls be used for each antibody - see  CAP checklist
 >> ANP.22550. They do not specify for each slide. Since positive QC's 
should be
 >> kept filed for the same number of years as the patient slide & records, 
 >> should be possible to pull a QC slide from the IHC run for a particular
 >> slide. In the CAP comment on ANP.22550, the use of internal QC's is also
 >> mentioned. Although there are many ways of dealing with the issue of 
 >> I'm sure we all want to do what is prudent, abide by the regulations, 
 >> increase the level of patient care.
 >> Just my 2 cents worth.
 >> Patti Loykasek
 >> Phenopath Laboratories
 >> Seattle, WA
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