Re: [Histonet] IHC QC's
Re: [Histonet] IHC QC's
I've always run one positive control for each antibody and a
negative control for each paraffin block. When I was the immuno supervisor at
AFIP (during another life) we would anywhere from 25-100 cases of the same
antibody, i.e. we would run 1 CD45 control and 50 patient slides, but each
patient slide would have 1 negative. I haven't had a problem yet,
including both the CAP and CLIA inspections I went through this
year.
I have to agree with Patti, since I work in
a reference lab, we don't receive the good cases that a hospital would so we
have to make do with what we can get.
Joe Nocito BS, HT (ASCP) QIHC
Histology
Manager
Pathology Reference Lab
San Antonio, Texas
----- Original Message -----
Sent: Monday, October 13, 2003 2:18
PM
Subject: Re: [Histonet] IHC QC's
There was in fact, a post from Nick
Kirk on running a positive control with each case. I do realize the CAP
requirements and am familiar with the checklist.
Patti
Loykasek
Phenopath Laboratories
Seattle, WA
I don't
think anyone said a POSTIVE control should be run with each slide.
We were talking about negative controls, I believe.
I
just copied and pasted from the latest CAP survey in another
email.
Hazel
Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's
Hospital
Phone - 501.364.4240
Fax - 501.364.3912
-----Original Message-----
From: Patti Loykasek
[mailto:ploykasek@phenopath.com]
Sent: Monday, October 13, 2003
10:52 AM
To: histonet
Subject: [Histonet] IHC
QC's
I'm glad that everyone is so
concerned with both negative and positive IHC controls. There is certainly
more than one side to this issue. I will say that I don't think a positive
QC on every slide is absolutely necessary, for many reasons. If the QC is
rare & precious, then it is a waste of resources. As is running a
negative control for every possible technique permutation on small amounts
of tumor. I would rather have slides with tumor left for additional
studies than have wasted tumor sections on 4-6 negative controls. You can
always evaluate non-specific staining on slides that have had an antibody
applied & that are negative with that antibody. The CAP is specific
that positive controls be used for each antibody - see CAP checklist
ANP.22550. They do not specify for each slide. Since positive QC's should
be kept filed for the same number of years as the patient slide &
records, it should be possible to pull a QC slide from the IHC run for a
particular slide. In the CAP comment on ANP.22550, the use of internal
QC's is also mentioned. Although there are many ways of dealing with the
issue of QC's, I'm sure we all want to do what is prudent, abide by the
regulations, and increase the level of patient care.
Just my 2 cents
worth.
Patti Loykasek
Phenopath Laboratories
Seattle, WA
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