Hello Paula,
I use Brst-2 from Signet cat.# 611-23
It is ready to use but I dilute it further @ a 1:1 dilution and
incubate for 15 minutes
I pretreat for 5 minutes @  room temperature with Dako's Prot. K cat.#
3020 (they have small and large volume)
The detection system that I use is Dako's LSAB2 kit, but other kits
work just as well.
Good Luck
Dana Settembre
University Hospital-UMDNJ
Newark, NJ

>>> Paula Wilder  10/1/2003 4:39:15 PM >>>
Can anyone tell me how they handle this antibody?  We are getting quite
a 
mixed response from neighboring institutions.  Thanks so much!
Paula Wilder
St. Joseph Medical Center
7601 Osler Drive
Towson, MD.

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