Re: [Histonet] BRST2

From:Dana Settembre

Hello Paula,
I use Brst-2 from Signet cat.# 611-23
It is ready to use but I dilute it further @ a 1:1 dilution and
incubate for 15 minutes
I pretreat for 5 minutes @  room temperature with Dako's Prot. K cat.#
3020 (they have small and large volume)
The detection system that I use is Dako's LSAB2 kit, but other kits
work just as well.
Good Luck
Dana Settembre
University Hospital-UMDNJ
Newark, NJ

>>> Paula Wilder  10/1/2003 4:39:15 PM >>>
Can anyone tell me how they handle this antibody?  We are getting quite
mixed response from neighboring institutions.  Thanks so much!
Paula Wilder
St. Joseph Medical Center
7601 Osler Drive
Towson, MD.

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