| From: | Carole Fields |
Hi Celia,
We also had problems with gastric bx's and it wasn't a consistent problem. I retrieved them and then stained them H&E and they were much better. It did not seem to matter what you retrieved them with either....very weird. We think it is something with the fixation. I had a problem with bone marrows and it turned out to be, the decal was messed up, but I retrieved them and they stained better. Go figure.... but it worked. My Pathologist also heard about this at a conference he went to. They did not know why it worked either. We do the microwave Steiner now and it works much better. Try the retrieval...good luck.
Carole Fields
Lexington Medical Center
W.Columbia, SC
-----Original Message-----
From: celia [SMTP:cfarm@sio.midco.net]
Sent: Wednesday, October 08, 2003 6:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] warthin starry
Hello Everyone,
I have put this question out once before and I didn't get much for a response so I thought I would try putting it out there one more time. I have an ongoing problem with my Warthin-Starry stain. The peripheral edges of some of the gastric biopsies are not staining. I am also experiencing this same non-staining with the Steiner stain on the Ventana stainer. I have tried reducing the temp on the processor down from 60c to 58c thinking they might be "cooking" on the processor (the H&E's look fine) as well as checking the ph of my solutions. This did not work. Could this be a handling problem prior to fixation in 10% NBF? I am at my wits end. Any suggestions will be greatly appreciated.
Thanks in advance.
Celia, HT
SVH, Sioux Falls, SD
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