RE: [Histonet] negative IHC controls
only should you be running a negative control for each patient
slide. That negative control should be treated just as your antibody
is. If the antibody is rabbit and antigen retrieved, so should your
control. If another antibody on the same patient is mouse and not
retrieved another negative control should be run with this same
protocol. In the United States, labs that are inspected by the CAP,
are required to run these controls. MONEY should never be
considered as a reason to stop doing a part of a procedure. It's
poor patient care and lousy quality control.
Hazel Horn, HT/HTL (ASCP)
Phone - 501.364.4240
Fax - 501.364.3912
I would like to get a feel for how many out there
are running negative control slides for IHC.
In our lab we do just a handful of antibodies and
initially I had been running a negative control slide with each patient slide.
After much discussion with our pathologists, we decided to omit these
negatives (which were conistently negative) and continue to just run a
positive control with each primary antibody for the run. We use the Dako
autostainer and prediluted primaries. The decision to stop running
negatives also coincided with Dako's decision to sell the negative control
sera separately from the primaries (they used to come packaged
together). Perhaps I assumed that discontinuing to pair these reagents
together meant that few labs were using the negatives.
Anyhow, after having reviewed the last QMPLS
(Canada) survey committee comments, I believe the committe would like a
negative control run with each patient tissue slide in order to evaluate
background (they have used NCCLS guide pages as reference).
Incidentally we weren't a part of the survey due to a
Any help or advice would be
The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Arkansas Children's Hospital
<< Previous Message | Next Message >>