RE: [Histonet] negative IHC controls

From:"Horn, Hazel V"

Not only should you be running a negative control for each patient slide.   That negative control should be treated just as your antibody is.   If the antibody is rabbit and antigen retrieved, so should your control.   If another antibody on the same patient is mouse and not retrieved another negative control should be run with this same protocol.   In the United States, labs that are inspected by the CAP, are required to run these controls.    MONEY should never be considered as a reason to stop doing a part of a procedure.   It's poor patient care and lousy quality control.   IMHO.
Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital

Phone - 501.364.4240
Fax - 501.364.3912
-----Original Message-----
From: vermast []
Sent: Wednesday, October 08, 2003 3:57 PM
Subject: [Histonet] negative IHC controls

I would like to get a feel for how many out there are running negative control slides for IHC. 
In our lab we do just a handful of antibodies and initially I had been running a negative control slide with each patient slide.   After much discussion with our pathologists, we decided to omit these negatives (which were conistently negative) and continue to just run a positive control with each primary antibody for the run.  We use the Dako autostainer and prediluted primaries.  The decision to stop running negatives also coincided with Dako's decision to sell the negative control sera separately from the primaries (they used to come packaged together).  Perhaps I assumed that discontinuing to pair these reagents together meant that few labs were using the negatives.
Anyhow, after having reviewed the last QMPLS (Canada) survey committee comments, I believe the committe would like a negative control run with each patient tissue slide in order to evaluate background  (they have used NCCLS guide pages as reference).  Incidentally we weren't a part of the survey due to a technicality.
Any help or advice would be appreciated.
L. Vermast
Stratford, Ont.

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