RE: [Histonet] burned artifact

From:Tony Henwood


My suggestions are:
Don't let the fixation in NBF be too short.
Use a more gentle processing (less pressure & vacuum, lower temperature,
shorter time in alcohols and xylene).

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Millicent Bruce []
Sent: Friday, 17 October 2003 1:47
Subject: [Histonet] burned artifact

For the past couple of years we have been getting a
burned artifact in our biopsies.  The pathologist have
said that on the slides the nucleus look smudged or
burned.  It is mostly on the small bxs, but we have
seen it on larger specimens also.  We have bought a
new tissue processor, and a new slide stainer thinking
that might be the problem.  We are still seeing the
artifact.  Is there anybody that might can tell us
what is causing the problem?  We have tried everything
we can think of.

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