RE: [Histonet] Superfrost Plus Slides

From:"Favara, Cynthia (NIH/NIAID)"

I would be much more inclined to think there is something amiss with the
vortex mixers and or the rinses on the Nexus. I use the Nexus and am
generally pleased with the results for high volumes but from my experience
one clogged vortex mixer can cause erratic and uneven staining. One way to
access this would be to run duplicates staining by hand and see if you get
the same variation with the slides. I do think in terms quality of staining
that staining by hand when done consistently beats any machine hands
down!!!! or up!!!!!


Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

-----Original Message-----
From: Brennan, Liam []
Sent: Thursday, October 02, 2003 8:10 AM
To: 'Bill Sinai'; 'HISTONET'
Subject: RE: [Histonet] Superfrost Plus Slides

Dear Bill

We have experienced uneven staining on our Ventana NexES stainer over the
past number of months. It is not confined to any particular antibody, and
does'nt happen all the time. We have been told by Ventana that it is a
worldwide problem with coated slides. Apparently a number of brands are
manufactured in the same factory. We use Starfrost slides from Knittel
Glaser in Germany.
As yet, working with the people from Ventana, and trying a variety of slide
brands, we have been unable to resolve the problem.
I would be interested in hearing from others that have encountered similar
problems, and what actions they have taken to resolve the issue.

Liam Brennan
Histopathology Dept
Belfast City Hospital
Northern Ireland

-----Original Message-----
From: Bill Sinai []
Sent: 01 October 2003 22:31
To: histonet (E-mail)
Subject: [Histonet] Superfrost Plus Slides

Dear All,

Has anyone had a problem with Superfrost Plus slides on automated
Immunohistochemistry Staining machines?  If so what have the problems been?
Also if the problem was resolved and how it was resolved.

We obtain our slides through a third party agency in Australia.  We are at
present using Menzel-Glaser (German) brand.  I am led to believe they are
the largest slide and coverglass manufacturer in the world.

Bill Sinai
Laboratory Manager
Tissue Pathology, ICPMR
Westmead NSW 2145
Ph 02 9845 7774


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