RE: [Histonet] Shattering Mouse Brain

From:"Barbara Stancel"

Dear LaTricia,

Although it sounds like a problem in processing, reprocessing may not be the 
answer you need right now. Your best bet at this time is to lower the 
temperature of the water bath in 5 degree increments until you get to a 
temperature where the tissues does not explode. Of course that may mean you 
have to leave the section on the water bath a few minutes to relax bumpy 
areas. Best of luck.

Histologically yours, Barbara


Barbara H. Stancel, HTL(ASCP)HT
USDA, FSIS, OPHS, Eastern Laboratory, Pathology
Athens, Georgia  30604






-----Original Message-----
From: LaTricia Faison [mailto:lfaison@mail.mcg.edu]
Sent: Wednesday, October 08, 2003 12:27
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Shattering Mouse Brain


Does anyone know what may cause mouse brain to separate when put on the
water bath?  The tissue was given to me after it had been in
paraformaldehyde for about 7-8 months.  It was paraffin processed and
shattered soon after touching the water bath.

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