RE: [Histonet] Sentinel nodes

From:"DeLovino, Salvacion S."

After the first H&E cut, we cut another for IHC. Then we go 40 microns deeper then cut another set of H&E/IHC slides. Then we go another 40 microns deeper and cut the final set. So we have a total of 6 slides. That's if we have a big enough tissue. If not, then just adjust how deep we go depending on the size or thickness of the specimen.


Salvacion Delovino


-----Original Message-----
Sent: Wednesday, October 08, 2003 1:35 PM
To: histonet@lists.utsouthwestern.EDU
Subject: [Histonet] Sentinel nodes


Good afternoon everyone,

We routinely cut our negative sentinel node biopsies shallow, cutting 5 levels (mounting levels 2 and 4 for IHC), approximately 30 microns between levels.  Now, a pathologist read that "most institutions" are cutting them deeper, approximately 100 microns between levels.  This is totally an INFORMAL poll, but I would appreciate any feedback from the community.  I will check the archives as well, but thought I would get the question out there before everyone heads off to Kentucky:-)  Thanks.  Jennifer

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