RE: [Histonet] Purple Haze.....
I can't see how the
temperature of the water on the stainer could be causing this artefact as the
problem tends to be intermittent even when batch staining.
Sometimes within a rack
of slides you will get some slides from GI biopsies showing the artefact while
others from the same clinic session do not.
It has to be something
individual to each specimen in my opinion, otherwise we would see every slide
going through on that particular batch exhibiting the same staining artefact,
which we don't.
I still think the actual
biopsy process has a major part to play, especially as that's the only variable
we don't have any control over and all the others we do.
The temperature thing is
a spurious argument in my opinion when you consider all blocks are chilled prior
to cutting so everything is cold at some point and if you do immuno antigen
retrieval you apply excessive heat to the slide and still get good nuclear
detail when the section is counterstained.
I think it has to be
Check the temperature of the water on your stainer.
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