RE: [Histonet] Purple Haze.....
From: | "Yaskovich, Ruth A (NIH/NIDCR)" |
I used to get this problem once in awhile and haven't had it since switching
to charged slides. May-be in the amounts of gelatin? What kind of
hematoxylin are you using?
Ruth Yaskovich
N.I.D.C.R.
N.I.H.
-----Original Message-----
From: Therersa Stegall [mailto:STEGTM@samcstl.org]
Sent: Wednesday, October 08, 2003 1:50 PM
To: KMMerril@LancasterGeneral.org; histonet@pathology.swmed.edu
Subject: [Histonet] Purple Haze.....
Kathleen:
Thanks for naming the amorphous blight that has been plaguing us lately.
This "Jimi Hendrix" artifact has been running 'round our brains for the past
few weeks. We have 1-started filtering the hematoxylin each morning,
2-alternated between Sta-on, gelatin, and naked waterbaths, 3-scrubbed the
entire staining system, 4-decreased time in microwave, ovens, for drying
slides; and yet, we still find this on some biopsies. Not always all the
biopsies in a batch, this led us to ask GI about collection procedures, and
possible air-drying, they insist it's not them. We've checked pH on water,
accurately calibrated the bluing and acid/water washes (we stain
regressively), and just about run out of ideas. I'm beginning to suspect
the hematoxylin is leaving a precipitate, or maybe the microtomy is
inconsistent and ruining cell
integrity, or .... maybe it's terrorism! Any ideas out there?
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>