RE: [Histonet] Need silver stain for granules in adrenals
you are trying to demonstrate are Chromaffin cells of the adrenal glands so a
Masson-Fontana should work, if not try a Schmorl or carry out the Chromaffin
use Iodate oxidation you can distinguish between Adrenaline and noradrenalin
fairly easily as iodates oxidise noradrenaline much quicker than they do
Could anyone suggest a stain for noradrenalin and adrenalin granules in
paraffin embedded adrenals. We were asked to do a gremilius stain and it
did not stain the granules. Are these slides lost or can we re stain
them? We usually do a recut of the block, but it is asked if we could
use the same tissue slide. The stain actually worked, but not for the
From what we read a Fontana-masson should work. There probably is a
better stain our there for this specific target. Can anyone with some
experience with staining these granules share their staining protocol.
At this time the request is not for
Thanks for your help.
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