RE: [Histonet] Mouse Embryo Processing

Hi Stephanie,
It depends on what you are looking for.  If you are interested in multiple organs the most consistent way of processing is trimming the embryo into three mid-siggital sections for gestational days 15-18.  Two on either side of the umbilicus and the umbilicus section.  I have processed a lot of embryos and found this is the most consistent reproducible processing method.  I would also agree that you need more time in the xylene, we use 3 stations for 45 min each.

Jason D. Burrill, B.A., HT(ASCP)
Senior Supervisor, Histology
Charles River Laboratories
251 Ballardvale Street
Wilmington, MA 01887
ph:   978-658-6000 ext 1652
fax:  978-988-8793

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