Try 1:10 for ER and 1:150 for PR with HIER pH 6.0 20 minutes steam, 10
min cool down
Rena Fail

-----Original Message-----
From: histonet-admin@lists.utsouthwestern.edu
[mailto:histonet-admin@lists.utsouthwestern.edu] On Behalf Of THERESA
ROHR
Sent: Thursday, October 02, 2003 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] DAKO ER and PR antibodies


Hi out there in Histonet land:

I am having a problem working up dilutions for DAKO's ER (M7047) and PR 
(M3569) antibodies.
I am using the Envision+ Detection system

Suggestions from DAKO were:

ER  1:100 to 1:150 with Envision+ and a 20 minute target retrieval PR
1:200 to 1:400 with Envision+ and a 20 minute target retrieval.

I've gone less and more and still cannot get any where near the staining
on 
these cases that went out to a IHC Laboratory than I am using as a 
comparison.

Any suggestions would certainly be appreciated.

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