[Histonet] warthin starry
I have put this question out once before and I
didn't get much for a response so I thought I would try putting it out there one
more time. I have an ongoing problem with my Warthin-Starry stain.
The peripheral edges of some of the gastric biopsies are not staining. I
am also experiencing this same non-staining with the Steiner stain on the
Ventana stainer. I have tried reducing the temp on the processor down from
60c to 58c thinking they might be "cooking" on the processor (the H&E's look
fine) as well as checking the ph of my solutions. This did not
work. Could this be a handling problem prior to fixation in 10% NBF?
I am at my wits end. Any suggestions will be greatly
Thanks in advance.
SVH, Sioux Falls, SD
<< Previous Message | Next Message >>